Pegylated Mechano Growth Factor (PEG MGF)
Pegylated Mechano Growth Factor (PEG
MGF) is a variant of the IGF-1 (insulin-like growth factor-1), which stimulates
myoblasts division and allows for muscle fibers to fuse and mature. This is a
process required for growth of adult muscle. The difference between IGF-1 and
MGF is that MGF stimulate myoblasts division through stimulation of different
receptors.
At early stages, the use of MGF was limited because, once in the body, this
drug broke down within a few minutes. But the scientists found an easy and
brilliant decision, which was pegylation. A MGF molecule was attached to a
polyethylene glycol molecule, which acts as a protective coating and allows MGF
to be carried through the blood stream without being broken down without
loosing its bioactivity and efficiency.
Pegylated MGF is more bioavailable. Injected 2-3 times per week, it
maintains effective concentration for a long period.
In bodybuilding this drug is used to accelerate muscle growth. Other effects
of the drug include decrease of fat mass of the body, endurance increase,
improvement of immune defense and skin, bone strengthening, lowering of
cholesterol level, accelerated recovery. Those who administered MGF declared
that they experienced no side effects, which frequently occur with the use of
other drugs for gaining body mass.
Pegylated Mechano Growth
Factor dosage regimen:
The average dose is 1000 mcg per 24 hours; the frequency is 2-3 times per
week. The drug is injected subcutaneously 1-2 hours before training to imitate
physiologic secretion. The cycle duration is 5-6 weeks.
PEGylated Mechano Growth Factor (PEG-MGF)
PEGylated Mechano Growth Factor (MGF)
Russianstar
" Experiences with MGF and PEG MGF a complete guide.
This article will discuss the use of Mechano growth factor and clear up a few
myths.
WHAT IS MGF?
Mechano Growth Factor (MGF) also known as IGF-1Ec is a growth factor/repair
factor that is derived from exercised or damaged muscle tissue, Its called MGF
as IGF-1Ec is a bit of a mouthful and harder to identify amongst the other igf
variants.
What makes MGF special is its unique role in muscle growth.
MGF has the ability to cause wasted tissue to grow and improve itself by
activating muscle stem cells and increasing the upregulation of protein synthesis,
this unique ability can rapidly improve recovery and speed up muscle growth.
MGF can initiate muscle satellite (stem) cell activation in addition to its
IGF-Ireceptor domain which ithen in turn ncreases protein synthesis turnover,
and therefore can if used correctly improve muscle mass over time.
The liver produces 2 kinds of MGF splice variants of igf..
1) IGF-1Ec This is the first phase release igf splice variant and it appears to
stimulate satellite cells into activation, This is the closest variant to
synthetic MGF.
2) liver type IGF-IEa this is the secondary release of igf from the liver, and
its far less anabolic.
MGF differs from the second variant IGF-IEa as it has a different peptide
sequence which is responsible for replenishing the satellite cells in skeletal
muscle, in other words it is more anabolic and longer acting than the
systematic release of the second MGF liver variant.
So just think of MGF as a highly anabolic variant of igf. After you have
trained, the IGF-I gene is spliced towards MGF then that causes hypertrophy and
repair of local muscle damage by activating the muscle stem cells as well as
other important anabolic processes, including the above mentioned protein
synthesis, and increased nitrogen retention.
In rats some studies have shown muscle mass increases of 20 percent from a
single mgf injection.. somewhow i think many of these studies are not accurate,
however the potential is undeniable.
HOW TO USE MGF
Now when you train what happens to your muscles, they break down, the cells are
damaged, muscle tissue needs to be repaired and your body produces 2 forms of
MGF splice variant, The first initial release of the above mentioned number 1
variant from the liver helps muscle cell recovery, if there is no MGF then
muscle cells die, thats the large and small of it.
As muscle is a post-mitotic tissue and as such cell replacement is not a means
of tissue repair , If the cells are not repaired they die and your muscles get
smaller and weaker.
The muscle The pool of these stem cells is apparently replenished by the action
of MGF, which is produced as a pulse following damage.
Now with synthetic injections of MGF you can increase the pulse and so speed up
recovery, and increase the muscle tissue cells by stimulating satelite cells
into full maturity. 200mcg bi lateraly is the very best choice of dosing in
muscles trained.
Thew only problem with MGF and this is the reason i dont like it, is that it
has such a short half life, just a few minutes, between 5-7, and it needs to be
used immediatley post workout as it wont work if muscle tissue hasnt been
damaged, thats why for me personaly i think the best option is PEG MGF.
Nevertheless MGF has a huge role to play, and is administered to those with
muscle wasting diseases and for those who are elderly and have lost muscle mass
for good reason, it is EXTREMELY anabolic.
HOW TO USE PEG MGF
This is a very important section.
When using MGF thats pegylated thats the addition of Polyethylene glycol, its a
non toxic additive that increased the half life of MGF from minutes to hours.
This means its uses and versatility make it a tremendous addition to a
bodybuilders aresnal.
I have found it most effective as its effects are systematic, that means they
have a whole body effect wherever muscle has been damaged or is diseased.
The next aspect we need to look at is how to make the most use of a long acting
version of MGF.
When your muscle is damaged your body releases a pulse of an MGF splice variant
as i outlined above, followed by a less anabolic longer acting version from the
liver... So it seems a waste to inject MGF at this time as you will just blunt
your bodys own release, your not enhaning it.
So using PEG MGF on non workout days is actualy the very best route, the muscle
has been damaged, so there are plenty of receptors for MGF, the effects are
systematic so all muscles will be helped to recover through increased nitrogen
retention, protein turnover, and satelite cell activation. Recovery is just
going to sky rocket.
Doing this means your increasing the length of your bodys own mechanism for
muscle repair and growth, your opening up the anabolic window.
NOW PLEASE TAKE CAREFUL NOTE.
Running PEG MGF in synergy with IGF is perfect but there are things you need to
know.
If you dose them at the same time, as IGF has such strong receptor affinity,
The effectivness of MGF will just be wasted.
The best option and the very best choice i feel is this....
IGF DES on workout days Pre workout, or IGF1-LR3 this wont blunt your bodys own
MGF release from the liver, and whereas IGF1-LR3 has a more systematic effect
and only a very small localised anabolic effect, DES on the other hand is verty
anabolic in a localised way, so bring up lagging muscle parts with DES, and
then the following day Dose MGF at 200-400mcg subq to increase recovery and the
mechanism for growth. Perfect synergy.
Over a 4 week run i noticed about 4lb increase with the PEG MGF and DES
partnership. And roughly the same weight in fat loss, very impressive, some
though have noted far greater increases in muscle mass.
If your on an AAS cycle there is no need for the addition of DES as IGF levels
will already be elevated, then the addition of PEG MGF can take your recovery
and gains to a new level.
STORAGE ETC
Dosing 3 times a week is best, and 1ml of BA water for every 2mg is optimal.
Storage in the fridge for up to 6 months. Avoid exposure to heat or sunlight.
According to RS nice protocol of IGF DES and PEG MGF will be
IGF DES during workout 80mcg and above ( about 4X week)
PEG MGF next day of workout of day 200-400 mcg at 2 pm (about 3X week)
but for beginners
50mcg IGF DES and 200mcg PEG MGF is sufficient
My bro got nice results with these peptides
Gained about 8 pounds pure muscle mass in just 5 weeks
__________________
Current cycle
week 1-16 test e 500mg/week
week 1-14 deca 400mg/week
Quick summary: MGF is a splice variant of the IGF produced by a frame shift if
the IGF gene. MGF increase the muscle stem cell count, so that more may fuse
and become part of adult muscle cells. This is a process required for adult
muscle cells to continue growing.
Why PEGylate MGF?
MGF exhibits local effects in skeletal muscle and without modification is not
systemic (can’t travel through the body). The problem with synthetic MGF is
that it is introduced IM and is water based so it goes into the blood stream.
MGF is not stable in the blood stream for more than a matter of minutes.
Biologically produced MGF is made locally and does not enter the bloodstream
and is short acting so stability is not an issue. By PEGylating the MGF we can
make synthetic MGF injected IM almost as efficient as local produced MGF.
Clinically proven Advanced Pegylation, the technology of polyethylene glycol
(PEG) conjugation, holds significant promise in maintaining effective plasma
concentrations of systemically administered drugs. It does this by surrounding
part of the peptide with a unique structure made of polyethylene glycol, which
can be attached to a protein molecule. The result of a correct PEGylation is
simlar to the protective mechanism of a turtle shell. The polyethylene glycol
groups protect the peptide but don’t surround it completely. The active sites
of the peptide are still free to do their biological function. In this case the
shell is a negative charged shield against positively charged compounds that
would affect the protein. This also provides a nice steric chamber for the
peptide to reside in. So it’s a happy turtle
Neurological research has shown that utilizing PEGylated MGF resulted in a
longer more stable acting version of the MGF peptide in serum/blood.
Bottom line
PEGylation can improve performance and dosing convenience of peptides,
proteins, antibodies, oligonucleotides and many small molecules by optimizing
pharmacokinetics, increasing bioavailability, and decreasing immunogenicity and
dosing frequency. PEGylation also can increase therapeutic efficacy by enabling
increased drug concentration, improved biodistribution, and longer dwell time
at the site of action. As a result, therapeutic drug concentrations can be
achieved with less frequent dosing—a significant benefit to patients who are
taking injected drugs.
The PEG itself does not react in the body and is very safe. PEG has been
approved by the US Food and Drug Administration (FDA) as a base or vehicle for
use in foods and cosmetics and in injectable, topical, rectal and nasal
pharmaceutical formulations. PEG has demonstrated little toxicity, is
eliminated intact by the kidneys or in the feces and lacks immunogenicity. The
risk associated with current PEGylated drugs are due to the way the drug itself
acts not the PEG. MGF, as it is being currently sold, is getting a bad rep from
people due to the fact they feel that they are not seeing gains from it. Many
people believe that the use of MGF in their cycles or protocols just flat out
won't work, however, this is far from the truth.
More MGF information
Complete Overview of MGF or IGF-IEc
From its sequence, MGF is derived from the IGF-I gene by alternative splicing
and has different 3' exons to the liver or systemic type (IGF-IEa). It has a 49
base pair insert in the human, and a 52 base pair insert in rodents, within the
E domain of exon 5. This insert results in a reading frame shift, with a different
carboxy (C) terminal sequence to that of systemic IGF-IEa. MGF and the other
IGF isoforms have the same 5' exons that encode the IGF-I ligand-binding
domain. Processing of pro-peptide yields a mature peptide that is involved in
upregulating protein synthesis. However, there is evidence that the
carboxy-terminal of the MGF peptide also acts as a separate growth factor. This
stimulates division of mononucleated myoblasts or satellite (stem) cells,
thereby increasing the number available for local repair
During the early stage of skeletal muscle development, myoblasts (muscle stem
cells) fuse to form syncytial myotubes, which become innervated and develop
into muscle fibres. Thereafter, mitotic proliferation of nuclei within the
muscle fibres ceases. However, during postnatal (after development) growth,
additional nuclei are provided by satellite cells (myoblast) fusing with
myotubules. Muscle damage-recovery seems to have a similar cellular mechanism,
in that satellite cells become activated and fuse with the damaged muscle
fibres (reviewed by Goldring et al. 2002). This is also pertinent to certain
diseases such as muscular dystrophy in which muscle tissue is not maintained
and which have been associated with a deficiency in active satellite (stem) cells
(Megeney et al. 1996; Seale & Rudnicki, 2000) and in myogenic factors
(Heslop et al. 2000). Skeletal muscle mass and regenerative capacity have also
been shown to decline with age (Sadeh, 1988; Carlson et al. 2001). The reduced
capacity to regenerate in older muscle seems to be due to the decreased ability
to activate satellite cell proliferation (Chakravarthy et al. 2000). The
markedly lower expression of MGF in older rat muscles (Owino et al. 2001) and
human muscle (Hameed et al. 2003) in response to mechanical overload has been
associated with the failure to activate satellite cells, leading to age-related
muscle loss (Owino et al. 2001). Your muscle cels can not grow once they have
reached a certain size unless they obtain more nuclei from the myoblast. MGF
increases the myblast available to donate their nuclei to the adult muscle
cell.
“MGF appears to have a dual action in that, like the other IGF-I isoforms, it
upregulates protein synthesis as well as activating satellite cells. However,
the latter role of MGF is probably more important as most of the mature IGF-I
will be derived from IGF-IEa during the second phase of repair. Nevertheless,
it has been shown that MGF is a potent inducer of muscle hypertrophy in
experiments in which the cDNA of MGF was inserted into a plasmid vector and
introduced by intramuscular injection. This resulted in a 20 % increase in the
weight of the injected muscle within 2 weeks, and the analyses showed that this
was due to an increase in the size of the muscle fibres (Goldspink, 2001).
Similar experiments by other groups have also been carried out using a viral
construct containing the liver type of IGF-I, which resulted in a 25 % increase
in muscle mass, but this took over 4 months to develop (Musaro et al. 2001).
Hence, the dual role MGF plays in inducing satellite cell activation as well as
protein synthesis suggests it is much more potent than the liver type or
IGF-IEa for inducing rapid hypertrophy.”
These results are based on actual transplantation of the DNA coding for the
peptides. This is a permanent effect and much more potent than IM injections of
the peptide itself. You will not see a 20% increase in muscle mass through IM
injections as claimed above.
PEGylated MGF dosing Protocols
The PEGylated version is going to be much longer lasting making a 1-2 dose per
week procedure possible. I still think its best used with IGF or AAS to
maximize the benefits so here are some sample protocols
Once a week PEG MGF/ IGF
Sunday 100-300 mcg MGF you can choose to site inject if you wish. I think
splitting large doses may benefit.
Monday –Fri IGF 50mcg e/d
Twice a week PEG MGF / IGF
Sunday and Wed MGF 50-150 mcg
MT, ThF IGF 50 mcg
These protocols are just to start as this is brand new feel free to tweak them
if you like. I will update them after we have done some testing.
MGF is a splice variant
of the IGF gene which increases stem cell count in the muscle and allows for
muscle fibers to fuse and mature. This is a process required for growth of
adult muscle. Natural MGF is made locally and does not travel into the
bloodstream. Synthetic MGF is water based and when administered
intramuscularly, travels into the bloodstream. MGF is only stable in the blood
stream for only a few minutes.
PEGylation is the act
of attaching a Polyethylene glycol (PEG) structure to another larger molecule
(in this case, MGF). The PEG acts as a protective coating and the theory here
is that this will allow the MGF to be carried through the blood stream without
being broken down.
Background
I have to be honest
here, and say that in my estimation, PEGylating MGF is basically something a
research chemical company did to have a bit of a market with no competition for
awhile. That’s not to say that it’s not a decent product, but honestly, in this
particular case, I feel that marketing was in the drivers seat with the
development of this version of MGF, and science was in the backseat asking “are
we there yet?”.
Action
MGF is produced
biologically when muscle fibers are broken down through resistance (weight
training). It is a potent factor in muscle growth. MGF stimulates muscle
growth, creates new muscle fibers, promotes nitrogen retention and increases
protein synthesis. This compound is commonly used for overall growth of muscle
and to promote growth in body parts that are not up to par with the rest of the
user's physique. Results usually depend on dosage. Fat loss and strength
increases are not typically seen with MGF's use (as they are in IGF-1 use).
The PEG itself is safe
for use as it is approved by the US Food and Drug Administration (FDA) and does
not react in the body. The PEG is not broken down in the body and excreted
(intact) through urine or feces. Any risk associated PEGylated drugs is due to
drug itself not the PEG per se.
Technical Data
In a study on older
rodents, muscle fiber reduction in their older muscles was found to be
attributed to decreased activity of satellite cells (1). After a certain size
was reached, growth ceased. In the presence of MGF, satellite cells became
activated and hypertrophy in mature muscles continued.
In experiments where MGF
was administered intramuscularly, there was a 20% increase in the weight of the
injected muscle fibers within 2 weeks (2). In further studies, it took 4 months
for IGF to cause a 25% increase in muscle mass (3). MGF was found to be more
potent than IGF-1Ea in rapid muscle growth (4).
[Note: This data is on
“regular” MGF, not the Pegylated version….we can assume similar results,
however]
User Notes
Although the science
looks impressive on paper, in the real world, we see something totally
different. While PEGMGF should have theoretically given the athletes who use it
better results than regular MGF, it struggles to provide even the same results
at a higher dosage (judging from the athletes I have personally spoken to).
So does that mean it’s
useless?
No, not at all. Not
entirely…
I think that the
PEGylation is actually a potentially useful addition to MGF if properly used.
If we assume that the PEGylation will extend the life of the MGF in the body
somewhat, then we can use it in a very specific manner to help our gains. It is
nowhere near as good as regular MGF though, and I wouldn’t use it unless I
really had the disposable cash on hand.
I feel that, based on
conversations with several athletes and bodybuilders, that PEGMGF is best used
in conjunction with (not instead of) regular MGF (and IGF). I feel that if one
were to use my Peptides protocol (to read about that in detail, check out the
article “Peptides: The Next Frontier in Hypertrophy”), I think that PEGMGF is
probably best used on off-days from training, to keep MGF levels elevated and
get additional hypertrophy from the longer releasing PEGMGF.
So, along with regular
MGF and Lr3IGF-1, if I felt it to be necessary, I might throw in some PEGMGF on
off-days from training, to get additional growth (and again, if it were me, I’d
probably recommend 400-500mcg of PEGMGF on off days, with a regular dose of
200mcg of regular MGF + 100mgs of Lr3IGF-1 on training days, as per my
article).
For most athletes I’ve
spoken to and worked with, this is what we’ve found to be optimal. Again,
though…I’m not very fond of this product, and it’s best used (if at all), as a
possible adjunct to an IGF + MGF cycle, and never in place of regular MGF.
Unfortunately, it just didn’t pan out as people hoped it would, but it’s not a
complete waste of money.